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1. Topic-
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2. Content-
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DNA Sequencing
Restriction Enzyme
Gel Electrophoresis
Base Pairing
DNA Polymerase
Polarity |
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3. Goals: Aims/Outcomes-
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1.Students will learn how restriction enzymes are used to cut DNA
sequences into smaller pieces.
2. DNA pieces are partially charged and move toward the negative end
(Polarity)
3. The fragments create a pattern that can be used comparison and
evidence evaluation. |
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4. Objectives-
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1. Students will examine crime scene evidence.
2. Students will model gel electrophoresis.
3. Students will draw a conclusion based on the DNA fingerprint evidence. |
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5. Materials and Aids-
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"Who Ate the Cheese" packet
Paper
Ruler |
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6. Procedures/Methods-
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A. Introduction-
| How long is our DNA? How can we make it smaller to analyze? |
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B. Development-
1. Review DNA sequencing from prior week.
2. Define the term Restriction Enzyme
3. Show class electrophoresis and briefly describe the purpose of
it. |
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C. Practice-
1. Hand out packets.
2. Find first RE cut together to model process. |
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D. Independent Practice-
1. Using the "Who Ate the Cheese" handout, complete procedure which
involves:
1. Finding the RE site on all 4 sequences and making cuts.
2. Setting up a paper electrophoresis model and gluing DNA pieces
on correct location based on polarity.
3. Drawing a conclusion by examining their paper DNA fingerprint. |
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E. Accommodations (Differentiated Instruction)-
No specific accommodations should be needed but I will have a model
for struggling students complete to help them.
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F. Checking for understanding-
| Students should be able to identify the suspect based on their completed
model. |
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G. Closure-
Teams will be dismissed based on answering questions related to
activity.
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7. Evaluation-
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| Students should find this activity easy to interpret if the steps
are followed properly. The warm-up on Thursday will include questions
addressing the 4 goals I have in mind from the lesson. Students will
simulate this in the lab setting at a later date. |
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