Subject: Medical Technology
1. Topic-
Biosafety Levels
 
2. Content-
Biosafety Level 1
Agents: Not known to consistently cause diseases in immunocompetent adult humans.
Practices: Standard microbiological practices
Safety Equipment: None required
Facilities: Open bench top, sink required

Biosafety Level 2
Agents: Associated with human disease. Hazard:
percutaneous injury, mucous membrane exposure, ingestion
Practices: BSL"�1 practices plus:-
- limited access-
- biohazard warning signs
- sharps precautions-
- biosafety manual defining waste decontamination or medical surveillance
policies

Safety Equipment: Primary barriers: Class I or II biosafety cabinets or other physical containment devices used for all manipulations of agents that cause splashes or aerosols of infectious materials; PPE: laboratory
coats, gloves, face protection as needed
Facilities: BSL"�1plus:-
- non"�fabric chairs and other
furniture easily cleanable
- autoclave available-
- eyewash readily available

Biosafety Level 3
Agents: Indigenous or exotic agents with potential for aerosol transmission; disease may have serious or lethal consequences
Practices: BSL"�2 practices plus:-
- controlled access-
- decontamination of all wastes-
- decontamination of
lab clothing before
laundering
-
- baseline serum-
Safety Equipment: Primary barriers: Class I or
II biosafety cabinets or other physical containment devices used for all manipulations of agents; PPE: laboratory coats, gloves, respiratory protection as needed

Facilities: BSL"�2 plus:-
- physical separation from access corridors-
- hands"�free handwashing"� sink-
- self"�closing double door access-
- exhaust air not recirculated-
- negative airflow into laboratory-
- eyewash readily available in lab

Biosafety Level 4
Agents: Dangerous/exotic agents which pose high risk of life"� threatening disease, aerosol"�transmitted lab infections; or related agents with unknown risk of transmission
Practices: BSL"�3 practices plus:-
- clothing change before entering-
- shower on exit-
- all material decontaminated on exit from facility
Safety Equipment: Primary barriers: All
procedures conducted in Class III biosafety cabinets
or Class I or II biosafety cabinets in combination with full"�body, air supplied positive pressure suit
Facilities: BSL"�3 plus:-
- separate building or isolated zone
- dedicated supply/exhaust, vacuum and decon system
 
3. Goals: Aims/Outcomes-
1. The students will be able to differentiate the 4 types of Biosafety Levels.
2. The students will be able to operate in the lab with accordance to the 4 types of Biosafety Levels.
3.Students will be able to identify the potential hazards located in the Clinical Laboratory.
 
4. Objectives-
1. To help the students understand the 4 types of Biosafety Levels.
2. To let the students know the potential hazard they can encounter in the laboratory.
3. To let the students know how to work efficiently in the laboratory.
4. To let the students identify the types of safety hazards.
 
5. Materials and Aids-
AV and visual aids.
 
6. Procedures/Methods-

A. Introduction-

1. The 4 types of Biosafety Level
2. The potential health hazards located in the laboratory.
3. How to protect yourself from health hazards in the laboratory.
 

B. Development-

1. Visual aid of the biohazard symbol and what it means.
2. A video presentation about the dangers in the lab.
3. Demonstration of proper handwashing.
 

C. Practice-

1. Students will be asked to identify potential health hazards according to their Biosafety Level.
2. Practical examination on how to do proper handwashing.
 

D. Independent Practice-

1. Homework: Identify the different Biosafety Level viral agents for each level.
2. Draw the different safety apparatuses used in the laboratory.
3. Illustrate on how to wear personal protective equipment.
 

F. Checking for understanding-

1. Quiz at the end of the lesson.
2. Question for clarifying things.
 

G. Closure-

1. Review of the Biosafety Levels.
2. Prayer
 
7. Evaluation-
Was able to properly teach the students about the Biosafety Level.
 
8. Teacher Reflection-
Overall, the lesson went pretty well. Students were able to do hands on experiment on proper handwashing. They were able to identify potential health hazards located in the laboratory.
 

This Lesson Plan is available at (www.teacherjet.com)